FastQCFastQC Report
Sun 3 Sep 2017
CAWHCACXX_l06n01_ogla_45.3510000009ba73.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWHCACXX_l06n01_ogla_45.3510000009ba73.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31616863
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG197200.016.7688085
GTATAGG137900.015.2297091
CTATACT198500.014.1936514
TATACCG102550.014.0843845
GTATTGG291650.014.0436131
GTATATA423750.013.6911361
GTATAAG146400.013.5016181
TAGACTG102200.013.2028415
TTATACT236150.013.1177574
GTATAGA170000.013.0527351
ACTGTGC141250.012.9455458
CCTATAC137400.012.7942513
CCTAGAC100750.012.7326443
GTATTAT234200.012.5787741
TAATACT251700.012.5527334
TCTATAC154950.012.479663
ATACTGT212250.012.3744976
TAGACAG111650.012.2981295
GTATTAG160150.012.2534021
GTATAAT223200.012.2407271