FastQCFastQC Report
Sun 3 Sep 2017
CAWHCACXX_l06n01_ogla_44.3510000009bad0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWHCACXX_l06n01_ogla_44.3510000009bad0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28372401
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG172750.015.5943315
GTATAGG126450.015.4815551
CTATACT187750.014.1459984
GTATATA385250.013.9864211
GTATTGG238800.013.9681541
TAGACTG90700.013.7244755
GTATAAG134450.013.6061771
TATACCG90100.013.2358155
GTATTAT217500.013.173291
GTATAGA152300.013.1034481
TACACTG128350.012.9190715
CCTATAC128550.012.78811553
TATACAG164450.012.3654995
TTATACT218450.012.266734
TCTATAC141350.012.1342763
GTATAAT210850.011.9436881
ACTGTGC125550.011.9164128
TACACCG91650.011.8196375
GTGTAGG117950.011.6422281
GTATAGT157800.011.5928421