FastQCFastQC Report
Sun 3 Sep 2017
CAWHCACXX_l06n01_ogla_41.3510000009bbec.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWHCACXX_l06n01_ogla_41.3510000009bbec.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25535953
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG168150.015.7950845
GTATAGG113050.015.0890361
GTATATA373500.014.3246951
GTATTGG223650.014.2132961
GTATAAG122500.013.6535061
TATACCG86500.013.51219755
TATACAG153400.012.9776395
CTATACT168850.012.662474
CCTAGAC80750.012.6503863
TTATACT210250.012.6323114
GTGTAGG103950.012.4788851
TACACTG115950.012.4568965
TAGACTG82400.012.4547065
TACACCG78300.012.378715
GTATTAT202650.012.2628861
ATAATAC194000.012.0740523
GTATAGA143900.011.9532341
TAATACT215600.011.9442134
ATTATAC195300.011.8720423
TATTGGG252050.011.8380672