FastQCFastQC Report
Sun 3 Sep 2017
CAWHCACXX_l06n01_ogla_40.3510000009bc64.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWHCACXX_l06n01_ogla_40.3510000009bc64.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25908432
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG167900.015.2240785
TATACCG85100.014.7950275
GTATTGG242400.014.7406451
GTATAGG114750.013.9957211
CTATACT167050.013.7941434
GTATATA343500.013.5836351
TATACAG152100.013.5568865
CCTATAC109750.013.2469643
GTATAAG123150.013.1954141
TACACCG86700.013.0423945
GTATTAG130600.012.98842051
TCTATAC135850.012.9751893
TAGACTG81200.012.93110755
GTATTAT191500.012.753391
TACACTG111200.012.6469675
TTATACT202950.012.5948394
TATTGGG267600.012.4282892
ACTGTGC114550.012.3971588
TGCGTTC165600.012.2178649
ATTATAC188600.012.16763
Produced by FastQC (version 0.11.2)