FastQCFastQC Report
Sun 3 Sep 2017
CAWHCACXX_l05n02_ogla_52.3520000009bb49.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWHCACXX_l05n02_ogla_52.3520000009bb49.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32648602
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG208300.016.2586125
GTATAGG141650.015.37126351
GTATTGG293650.015.3041611
GTATAAG157650.015.2441761
TATACCG103750.014.9249445
ACTGTGC141400.013.7644928
GTATATA450450.013.5194181
TACACTG144350.013.4582855
GTATAGA181350.013.1459511
TAGACTG101900.013.0516995
TTATACT255050.012.9431364
TATACAG184900.012.895855
CCTATAC142800.012.8553423
GTATATG296400.012.6000061
CTATACT211600.012.5579154
GTACTAG230250.012.4624271
TGCGTTC210800.012.430669
GGTGCGT222900.012.39479357
GTATTAT252400.012.3589991
GTCTAGG115800.012.3275231