FastQCFastQC Report
Sun 3 Sep 2017
CAWHCACXX_l05n02_ogla_51.3520000009bbcf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWHCACXX_l05n02_ogla_51.3520000009bbcf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24085142
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG109700.015.8665021
GTATTGG220300.015.4742991
GTATATA342850.014.5009221
TATACTG156900.014.4096545
TATACAG142500.014.3325395
TATACCG75700.014.1801935
TAGACTG77700.013.8763235
GTATTAG127050.013.6240771
GTATAAG109000.013.5863711
GTCTAGG85400.013.5124041
GTATAGA135450.013.276141
CTATACT158050.013.26030354
GTATTAT190700.013.2369471
TCTATAC127500.013.1211413
TATTGGG245850.013.1211322
CCTATAC106300.013.1149693
GTGTAGG102450.012.9062471
ACTGTGC109600.012.5604028
TACACCG78900.012.22048955
TACACTG109650.012.0421965