FastQCFastQC Report
Sun 3 Sep 2017
CAWHCACXX_l05n02_ogla_50.3520000009bc48.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWHCACXX_l05n02_ogla_50.3520000009bc48.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33634516
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG206800.016.8346735
GTATAGG144450.014.9641451
GTATATA463200.014.7568371
CTATACT219050.014.75431254
GTATTGG298900.014.1260221
TAGACTG107950.013.8152335
GTATAAG155150.013.7773321
TATACCG104250.013.7588495
ACTGTGC144300.013.6854778
TACACTG143650.013.522875
TCTATAC176750.013.3960413
TTATACT266200.013.3496814
TATACAG186550.013.1627475
CCTATAC146450.013.052173
GTATAGA183350.012.7848551
TAGACAG114500.012.7345655
CCTACAC133000.012.5304553
GTATTAG171050.012.4966561
TAATACT288450.012.4031764
GTATTAT260650.012.3473571
Produced by FastQC (version 0.11.2)