FastQCFastQC Report
Sun 3 Sep 2017
CAWHCACXX_l05n02_ogla_49.3520000009bcdb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWHCACXX_l05n02_ogla_49.3520000009bcdb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30434746
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG190750.015.8372045
GTATTGG273200.014.9326851
TATACCG97450.014.525145
GTATATA408450.014.29381751
GTATAGA170100.014.2093621
TAGACTG94150.014.1765965
GTATAAG140000.013.4202181
GTATAGG139050.013.1663341
GTATTAG157850.012.9680851
TATACAG176900.012.80785755
CTATACT195550.012.7741564
TCTATAC161150.012.4914533
GTCTAGG108000.012.4134161
TACACTG129250.012.3479445
TATTGGG315650.012.2831782
TTATACT237050.012.1590034
CCTAGAC98050.012.1515973
GTATTAT245300.012.1452091
ACTGTGC132650.012.0242958
CCTATAC133650.011.9702873