FastQCFastQC Report
Sun 3 Sep 2017
CAWHCACXX_l05n02_ogla_48.3520000009bd47.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWHCACXX_l05n02_ogla_48.3520000009bd47.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43505745
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG265450.016.8741555
GTATAGG194500.015.8828831
TATACCG141450.015.8165245
GTATTGG399350.014.6170781
GTATATA580100.014.1290881
GTATAAG195450.014.1095831
CTATACT280750.013.8745334
TATACAG254950.013.3584855
CCTATAC192650.013.3396653
GTATTAT315550.013.2613561
TACACTG198100.013.2597015
TTATACT330450.013.0232534
TCTATAC223250.013.0173963
ACTGTGC196750.012.787638
CCTAGAC141900.012.6266063
ATTATAC304100.012.5735193
GTATAGA239800.012.5218591
GTATAAT312050.012.4709281
TATTGGG449650.012.4030762
TACACCG138250.012.3345235
Produced by FastQC (version 0.11.2)