FastQCFastQC Report
Sun 3 Sep 2017
CAWHCACXX_l05n01_ogla_53.3510000009bab6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWHCACXX_l05n01_ogla_53.3510000009bab6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32855953
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG210400.015.6027495
GTATTGG276450.015.1418531
GTATAGG142150.015.04131
TAGACTG104150.014.6880925
CCTATAC150700.014.217793
TACACTG145700.014.1513855
GTATAAG156050.013.9755411
TACACAG148200.013.5920965
CTATACT226250.013.5857774
GTGTAGG138850.013.5509271
TATACCG108050.013.3664975
TACACCG111550.013.2878235
CCTAGAC109850.013.277223
TAGACAG119100.013.0438195
TATACAG192150.012.930945
TATTGGG310250.012.8015722
GTGCGTT203700.012.6377378
ACTGTGC147050.012.6291118
GGTGCGT207200.012.5617997
GTCTAGG123350.012.5573541
Produced by FastQC (version 0.11.2)