FastQCFastQC Report
Sun 3 Sep 2017
CAWHCACXX_l05n01_ogla_52.3510000009bb4c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWHCACXX_l05n01_ogla_52.3510000009bb4c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32648602
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG208950.016.188385
GTATTGG296800.014.6478141
GTATAGG147250.014.5847431
TATACCG104100.014.4212225
ACTGTGC140850.014.3651478
GTATAAG154350.014.1601171
GTATATA454150.013.92497351
TAGACAG117000.013.6432925
CTATACT211100.013.5704684
TACACTG145450.013.5223555
CCTAGAC105600.013.4065623
TTATACT260200.012.9815954
CCTATAC146900.012.9360973
TATACAG186000.012.8220225
TCTATAC172950.012.7456643
TAGACTG105150.012.6958785
GTATTAT252500.012.45696451
TGCGTTC210150.012.4079569
TACACCG103100.012.3492875
GTGTAGG130850.012.3458451