FastQCFastQC Report
Sun 3 Sep 2017
CAWHCACXX_l05n01_ogla_51.3510000009bbc2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWHCACXX_l05n01_ogla_51.3510000009bbc2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24085142
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG158600.015.9655555
GTATAGG105800.015.4033571
TATACCG76800.014.2274365
TATACAG146600.014.1290365
GTATATA348000.013.9396991
CTATACT161900.013.3219884
GTATTGG216450.013.2582521
TACACTG107600.013.1572175
GTATAAG112650.013.1170481
CCTAGAC80550.013.0932483
GTATTAG125350.012.7735761
CCTATAC109100.012.7585963
TACACCG79700.012.7560285
TAGACAG89700.012.7109745
GTGTAGG100550.012.6164191
TAGACTG76950.012.594525
TTATACT192550.012.4350364
GTCTAGG85300.012.2540751
TAATACT203650.012.2238194
ATACTGT166750.012.162456
Produced by FastQC (version 0.11.2)