FastQCFastQC Report
Sun 3 Sep 2017
CAWHCACXX_l05n01_ogla_50.3510000009bc4b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWHCACXX_l05n01_ogla_50.3510000009bc4b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33634516
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG201150.016.2722245
GTATATA461050.014.5508821
TATACCG102800.014.4643525
CTATACT219050.014.205154
GTATTGG305950.014.0384981
TAGACTG105050.013.747555
TATACAG191500.013.5943845
GTATAGG145000.013.4999411
GTATAAG151000.013.4040271
TCTATAC179400.013.1343013
GTATTAG172650.012.8514741
TAATACT289450.012.8345594
TTATACT273500.012.7840434
CCTATAC144100.012.7583563
TACACCG104900.012.725615
GTGTAGG142850.012.6720641
TACACTG149200.012.640655
ATAATAC259750.012.5463183
GTATTAT255900.012.3839221
GTATAGA187650.012.0773731
Produced by FastQC (version 0.11.2)