FastQCFastQC Report
Sun 3 Sep 2017
CAWHCACXX_l05n01_ogla_49.3510000009bcde.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWHCACXX_l05n01_ogla_49.3510000009bcde.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30434746
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG196250.015.8566535
GTATAGG139400.014.7592231
GTATTGG275800.014.4029141
GTATAAG143900.014.0335171
TATACCG90150.013.5440095
TACACTG133450.013.5283515
GTATATA415100.013.118081
TATACAG177950.013.0821145
ACTGTGC134000.013.0082798
TTATACT237650.012.9943764
GTATTAT242000.012.8017871
CTATACT198450.012.7840954
GTATAGA169450.012.5063811
CCTAGAC95900.012.434693
TAATACT251650.012.3469784
GTGTAAG92000.012.2921391
GTCTAGG105550.012.2897431
TCTATAC154700.012.2842753
CCTATAC133200.012.23403553
TATTGGG323100.012.11630252
Produced by FastQC (version 0.11.2)