FastQCFastQC Report
Sun 3 Sep 2017
CAWHCACXX_l05n01_ogla_48.3510000009bd4a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWHCACXX_l05n01_ogla_48.3510000009bd4a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43505745
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG279200.016.4374395
GTATAGG194300.014.8437031
GTATTGG388050.014.4974091
TATACCG140050.014.417115
CTATACT285300.014.3375134
GTATATA574000.013.9315221
CCTATAC190500.013.5168733
TACACTG192400.013.2599445
TATACAG246750.013.2273255
CCTAGAC140850.013.2221223
TAGACTG139950.012.8658585
GTATAAG199850.012.7434491
GTATTAG222900.012.7259631
TCTATAC220950.012.6431493
ACTGTGC198500.012.6337288
TATTGGG444500.012.5050672
GTATAGA234800.012.4857151
TTATACT335500.012.4046364
GTATTAT324450.012.3014951
GTCTAGG158850.012.0543621
Produced by FastQC (version 0.11.2)