FastQCFastQC Report
Sun 3 Sep 2017
CAWHCACXX_l05n01_ogla_47.3510000009bdc0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWHCACXX_l05n01_ogla_47.3510000009bdc0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26636322
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG168050.015.9161225
TATACCG88100.015.2068965
GTATTGG252700.014.6285231
GTATAGG118200.014.1096341
TAGACTG90100.013.8675065
GTATAAG123550.013.6909421
GTATTAT206850.013.598541
TATACAG160000.013.2725675
GTATTAG133100.013.1726881
CCTATAC114350.013.087053
TAGACAG95400.013.047295
TACACCG86550.013.0091425
GTATATA387000.012.9406391
TACACTG121800.012.7936355
GGTGCGT189100.012.7844477
ACTGTGC117150.012.6898878
GTATAGA152000.012.6601111
TCTATAC147150.012.6236193
CTATACT175400.012.5947864
GTCTAGG93000.012.5172591
Produced by FastQC (version 0.11.2)