FastQCFastQC Report
Sun 3 Sep 2017
CAWHCACXX_l04n02_obar_33.3520000009ba63.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWHCACXX_l04n02_obar_33.3520000009ba63.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43336239
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG280450.015.5688355
GTATAAG204450.015.2712141
GTATAGG191000.015.05281
TACACTG189000.014.8566785
GTATTGG354900.014.7426861
TATACCG136850.014.6855965
GTCTAGG142300.014.493731
CTATACT292450.014.4757694
GTATATA604350.014.169731
TACACCG135050.013.896285
CCTATAC198050.013.819693
GTGTAGG170900.013.6254031
TATACAG248750.013.56098655
TAGACTG139700.013.5357625
CCTAGAC133600.013.15969853
ACTGTGC178950.013.1109288
GTATAGA245800.013.1082011
GTATTAG226900.013.02720451
TCTATAC237050.012.9291633
TACACAG185650.012.9238665
Produced by FastQC (version 0.11.2)