FastQCFastQC Report
Sun 3 Sep 2017
CAWHCACXX_l04n02_obar_31.3520000009bb2f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWHCACXX_l04n02_obar_31.3520000009bb2f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37332443
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG254850.016.461675
TATACCG120950.016.1841225
GTATAGG166500.015.7834251
GTATTGG311850.015.710981
GTATAAG174250.014.8905331
CCTATAC169750.014.8638553
TACACTG173300.014.4206575
GTATATA522950.014.3486941
GTATAGA208600.014.3293571
TACACCG120900.014.265215
CTATACT250800.014.1895814
GTATTAG191950.013.8888131
TAGACAG133100.013.8857525
GTCTAGG137550.013.8539671
ACTGTGC166100.013.553228
CCTAGAC121600.013.4422393
TAGACTG118200.013.3449965
TAGACGG61850.013.2893655
GTGTAGG153000.013.2625441
TATACAG219300.013.1290075
Produced by FastQC (version 0.11.2)