FastQCFastQC Report
Sun 3 Sep 2017
CAWHCACXX_l04n02_obar_30.3520000009bba6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWHCACXX_l04n02_obar_30.3520000009bba6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36893083
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG242550.016.2187425
GTATAGG168800.015.5399691
GTATTGG329700.015.3502051
TATACCG115350.015.2807435
GTATAAG173350.014.4741671
GTATATA535750.014.0854861
CTATACT242600.014.0812994
GTCTAGG133600.013.8009441
ACTGTGC160850.013.7596748
TATACAG217450.013.6992345
TACACTG165800.013.697195
CCTATAC169950.013.5600083
GTATAGA205300.013.5178441
TAGACAG133000.013.5029395
TATTGGG375250.013.2956372
GTGTAGG154600.013.0943411
TCTATAC196700.013.0686973
TACACAG163000.012.9123195
TTATACT292900.012.8634794
TAGACTG119300.012.70393855
Produced by FastQC (version 0.11.2)