FastQCFastQC Report
Sun 3 Sep 2017
CAWHCACXX_l04n02_obar_29.3520000009bc11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWHCACXX_l04n02_obar_29.3520000009bc11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48662810
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTGG451400.016.2226221
TATACTG295100.015.6006465
GTATAAG222050.015.21593951
GTATAGG216350.014.7602031
TACACTG212550.014.7526775
TATACCG142650.014.65442855
TGCGTTC307650.014.264249
GTATAGA264700.014.2004631
GGTGCGT327550.014.0645237
GTGCGTT315250.013.9052648
TACACCG149850.013.7283755
GTATATA653650.013.696691
TAGACTG156500.013.6307575
CTATACT310200.013.3407434
TATACAG268400.013.2759855
TATTGGG512650.013.235652
GTCTAGG158850.013.2225451
TCTATAC247050.012.9824453
CCTAGAC151550.012.9802173
TTATACT363500.012.887724
Produced by FastQC (version 0.11.2)