FastQCFastQC Report
Sun 3 Sep 2017
CAWHCACXX_l04n02_obar_28.3520000009bc98.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWHCACXX_l04n02_obar_28.3520000009bc98.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29511937
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTGG253450.016.5746271
TATACTG188250.016.1774335
TAGACTG99300.014.8798225
TATACCG102650.014.8107695
GTATATA385800.014.5592511
GTATAAG139250.014.5377831
GTGTAGG132750.014.5336691
GTATAGG129450.014.5003611
ACTGTGC131850.014.4443918
CCTAGAC96700.014.3972663
TACACCG102750.014.3802085
CTATACT185350.014.354864
GTCTAGG105750.014.2000691
TCTATAC147000.013.8668823
TATACAG170500.013.7096815
CCTATAC129250.013.6022273
TAGACAG108500.013.5305465
TACACAG129700.013.5167735
TATTGGG286200.013.4811212
TAGACGG50450.013.2783625
Produced by FastQC (version 0.11.2)