FastQCFastQC Report
Sun 3 Sep 2017
CAWHCACXX_l04n02_obar_27.3520000009bd10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWHCACXX_l04n02_obar_27.3520000009bd10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38157983
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG240450.016.518615
GTATTGG346000.016.371391
GTATAGG174600.015.0238141
TATACCG123150.014.4673455
GTATATA517900.014.2039931
CTATACT237900.014.1599244
TATACAG231300.013.9677125
TAGACTG119650.013.937555
TATTGGG390450.013.8611692
GTATAAG167800.013.8201651
CCTATAC173850.013.7751833
TACACCG132550.013.5489015
GTCTAGG131550.013.4019641
TACACTG174900.013.3921145
GTATAGA207850.013.0548361
TCTATAC192050.012.9645823
GTATTAG193150.012.8674461
GTGTAGG161150.012.6801241
GGTGCGT260150.012.5785427
ATTGGGT380500.012.5752423