FastQCFastQC Report
Sun 3 Sep 2017
CAWHCACXX_l04n01_obar_33.3510000009ba66.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWHCACXX_l04n01_obar_33.3510000009ba66.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43336239
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG198400.016.1167051
TATACTG285700.015.8786585
TATACCG135600.015.4840035
CCTATAC197800.015.3456473
GTATTGG362350.015.3019041
CTATACT292000.014.7063964
TACACTG197400.014.2460755
TAGACTG139650.014.1165265
GTATAAG208250.013.8942511
GTATATA592700.013.8599911
TACACAG185800.013.857165
GTGTAGG176050.013.7907461
CCTAGAC134500.013.6678233
TATACAG253750.013.6284355
ACTGTGC184600.013.2247498
GGTGCGT249550.013.1896177
TAGACAG154350.013.1106325
TAGACGG70750.012.8912625
TGCGTTC238500.012.8846299
GTGCGTT254300.012.8685298