FastQCFastQC Report
Sun 3 Sep 2017
CAWHCACXX_l04n01_obar_32.3510000009bac3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWHCACXX_l04n01_obar_32.3510000009bac3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39133680
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG130200.017.8772815
TATACTG257050.016.4101185
TACACTG182800.015.6955715
GTATTGG357150.015.5243421
GTATAGG181300.014.7275831
CCTAGAC127600.014.5929993
GTATAAG198700.014.4660671
ACTGTGC172150.014.0983978
TAGACTG124750.014.0127595
CTATACT269450.013.9096184
TAGACGG63200.013.9050025
CCTATAC184800.013.8032543
GTATATA565600.013.5829331
CTACACT177000.013.3114334
TATACAG234550.013.3059635
TCTATAC214000.013.2960373
GTATAGA230100.013.2146691
GTCTAGG147200.013.104221
TAGACAG149100.013.0305295
GTGTAGG160250.013.0154591
Produced by FastQC (version 0.11.2)