FastQCFastQC Report
Sun 3 Sep 2017
CAWHCACXX_l04n01_obar_31.3510000009bb22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWHCACXX_l04n01_obar_31.3510000009bb22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37332443
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG248800.017.2410185
TATACCG121350.015.6191945
GTATAGG168100.015.319081
TATACAG218550.015.2367645
GTATTGG313350.014.9047591
TACACCG120100.014.6742685
TACACTG172550.014.5359845
TCTATAC199300.014.4913943
ACTGTGC159050.014.39370258
CTATACT251150.014.3749364
CCTAGAC124650.014.2906783
GTATAAG177400.014.1410431
GTATATA515500.013.6867061
CCTATAC168400.013.45523
TTATACT286900.013.3122364
TACACAG163050.013.1395725
TAGACAG133550.013.1252625
CTACACT164500.013.0815084
CCTACAC150350.012.8905253
GTGTAGG152300.012.8216241