FastQCFastQC Report
Sun 3 Sep 2017
CAWHCACXX_l04n01_obar_30.3510000009bba9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWHCACXX_l04n01_obar_30.3510000009bba9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36893083
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG242200.016.5531335
GTATTGG329350.014.8007191
GTATAGG171650.014.7251931
TATACCG116950.014.5003985
GTATAAG170100.014.3566121
TACACTG171450.014.3240215
CTATACT240200.014.2189784
TACACCG119350.013.8904065
TAGACTG119350.013.8506055
TATACAG214650.013.6984585
CCTATAC165450.013.4364863
GTATATA534050.013.3445051
TTATACT297100.013.2865094
TCTATAC196800.013.2752953
ACTGTGC158900.012.9424848
TATTGGG381800.012.8520092
TAGACAG132050.012.7703235
GTGTAGG150500.012.7537541
GTCTAGG132400.012.7030631
GTATAGA210050.012.553461