FastQCFastQC Report
Sun 3 Sep 2017
CAWHCACXX_l04n01_obar_29.3510000009bc14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWHCACXX_l04n01_obar_29.3510000009bc14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48662810
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG298800.016.342925
GTATTGG451150.015.0701771
TATACCG145100.014.862975
GTGTAGG193500.014.462191
TACACCG155750.014.3956435
GTATAAG227000.014.2534811
TACACTG212850.014.1269115
GTATAGG216000.014.0995091
CTATACT302650.013.9219514
GTATATA639950.013.6680781
TATACAG263500.013.5747395
CCTATAC217400.013.5687913
TAGACAG175650.013.5219395
TAGACTG150400.013.296935
CCTAGAC154100.013.1932023
GTATAGA272500.013.0417181
TACACAG208400.012.8785785
TTATACT364300.012.7655834
TGCGTTC308250.012.71178159
TCTATAC245000.012.6800443
Produced by FastQC (version 0.11.2)