FastQCFastQC Report
Sun 3 Sep 2017
CAWHCACXX_l04n01_obar_28.3510000009bc9b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWHCACXX_l04n01_obar_28.3510000009bc9b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29511937
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG190800.017.4023745
TACACTG140300.015.7436325
GTATAAG132550.015.5200421
GTATAGG134600.015.4248551
GTATTGG253900.015.212921
TATACCG104150.014.9141375
CCTATAC125450.014.8049493
TACACCG107150.014.6295665
TATACAG173850.014.5633795
CTATACT180200.014.3401534
ACTGTGC131200.014.1546178
TAGACTG98500.014.1299645
TAGACAG110300.013.8241735
ACAGTGC151950.013.7845668
GTGTAGG126500.013.5957371
GTCTAGG109200.013.4002481
GTATATA389600.013.2554761
TTATACT219550.013.1979374
CTACACT136400.013.0943254
TACACAG131650.013.0616295