FastQCFastQC Report
Sun 3 Sep 2017
CAWHCACXX_l04n01_obar_27.3510000009bd13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWHCACXX_l04n01_obar_27.3510000009bd13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38157983
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG246800.016.7259035
GTATTGG346850.015.7522771
GTATAGG176550.014.7737881
TATACCG121200.014.5799255
TACACTG184650.014.406345
GTATAAG173500.014.2119981
TACACCG128350.014.211845
CTATACT249100.014.1687014
TATACAG221550.013.9580325
TAGACAG138650.013.5329395
TATTGGG395150.013.4515472
CCTATAC173950.013.4079143
TAGACTG119400.013.367495
TCTATAC196400.013.3506293
ACTGTGC170900.013.28438958
GTGTAGG163600.013.2134231
GTATATA518200.013.1748781
TACACAG164000.012.8024585
GTATAGA207000.012.6005421
CCTAGAC122150.012.4829053