FastQCFastQC Report
Sun 3 Sep 2017
CAWHCACXX_l03n02_osat_150_2.3520000009bb99.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWHCACXX_l03n02_osat_150_2.3520000009bb99.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6829315
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG45100.023.1752665
TAGACCT22950.020.7024334
ATACTGT42700.020.5771736
ACTGTGC32650.019.6374958
TAGACTG22900.019.2941685
CTATACT59350.019.2930284
ACTGTGT35300.019.1050938
TACACTG32150.018.9150545
CTGGACT23400.018.8830074
TTATACT65500.018.6421454
TAATACT67700.018.5276054
CAGAGGT44700.018.3883444
CTAACCT33350.017.8081264
TAAGGCT29200.017.7356744
AATACTG38150.017.683675
GACTGTG22700.017.5747417
TTACCCT30850.017.5571384
CTACCCT26000.017.1774444
TAACCTG27450.016.9614245
TTACACT40200.016.9010644
Produced by FastQC (version 0.11.2)