FastQCFastQC Report
Sun 3 Sep 2017
CAWHCACXX_l03n02_osat_131.3520000009bb12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWHCACXX_l03n02_osat_131.3520000009bb12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13705220
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACTG45800.018.0480275
GTATAGG61950.017.7181381
ACTGTGC67900.017.2066868
TACACTG65200.016.466715
ACAGTGC73600.016.1967478
TATACTG77300.015.7328155
ACTGTCC47750.015.1182718
TACACCG51050.015.0752765
CTACACT67450.014.9323044
ACAGTCC39350.014.7247188
GTGTAGG63050.014.6959261
TCTACAC67100.014.3733353
CTGTGCA92600.014.2582339
GACTGTG43350.014.24249657
ACTGCGC38200.013.9247238
TGGACTG67500.013.6534995
CTGTGCG44550.013.6456479
ACACTGT68750.013.5401916
CCAGGAC62550.013.523
TACACAG60700.013.4612615
Produced by FastQC (version 0.11.2)