FastQCFastQC Report
Sun 3 Sep 2017
CAWHCACXX_l03n02_ogla_56.3520000009bce8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWHCACXX_l03n02_ogla_56.3520000009bce8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32727762
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG153100.017.7843911
TATACTG197400.017.5443445
CTATACT211700.016.090924
CCTATAC158850.015.7321163
GTATATA445450.015.0091031
TAGACTG109650.014.9474535
TACACTG147100.014.9205915
GTGTAGG126450.014.7308161
ACTGTGC143650.014.7125638
GTATAAG157050.014.5534761
TATACCG106700.014.1585725
GTATAGA178000.014.121971
TACACAG138700.014.0431365
CTACACT139050.013.94028954
TATACAG185450.013.6539135
TAGACAG115000.013.6324165
GTATTGG211150.013.6151951
GTCTAGG95350.013.5054241
CCTAGAC98150.013.456843
TCTATAC160950.012.9882393