FastQCFastQC Report
Sun 3 Sep 2017
CAWHCACXX_l03n02_ogla_55.3520000009bd60.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWHCACXX_l03n02_ogla_55.3520000009bd60.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38696651
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG239500.023.3919891
CCTATAC253650.020.9031833
CTATACT294600.020.2227944
TATAGGG220500.018.5953522
CCCTATA236200.017.9024432
TATACTG216650.016.555415
TAGACTG127150.016.4021155
TATACTA390550.015.7401665
GTATATA480750.014.7076241
GTATTGG253400.014.6829751
TACACTG177250.014.4998235
ATAGGGA252350.014.2143423
TATACCG129350.014.2133435
GTGTAGG160400.014.0421281
ACTGTGC177600.013.8521358
TCCCTAT264700.013.7150791
TATACAG219500.013.6783675
GACGTTA106850.013.53467120-21
TAGGGAT271800.013.4941094
ACAGTGC206400.013.2768798