FastQCFastQC Report
Sun 3 Sep 2017
CAWHCACXX_l03n02_ogla_54.3520000009bd7d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWHCACXX_l03n02_ogla_54.3520000009bd7d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32790644
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG207650.016.1062495
TATACCG97350.015.9087225
GTATAAG152350.015.8135411
GTATAGG146500.015.050261
TACACTG141150.014.3714525
GTATTGG278500.013.9058021
GTATATA436700.013.7757151
TATACAG183700.013.7580265
CTATACT202800.013.6343524
CCTATAC139450.013.4916413
GTATAGA179650.013.3046791
TCTATAC168600.013.300643
TAGACTG105550.013.1875185
CCTAGAC103850.013.1299643
GTCTAGG103350.013.0578441
GTATTAG168550.012.9967811
GTATTAT248650.012.8614591
TACACCG101600.012.7182945
GTATAAT242450.012.6023751
TAGACAG110200.012.5879475
Produced by FastQC (version 0.11.2)