FastQCFastQC Report
Sun 3 Sep 2017
CAWHCACXX_l03n01_osat_150_2.3510000009bb9c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWHCACXX_l03n01_osat_150_2.3510000009bb9c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6829315
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCT25500.022.1670364
TATACTG43900.021.4240475
CAGAGGT45000.021.1114644
TTATACT67900.020.917144
TAAGACT29950.020.7766424
TACACTG30000.020.4253435
CTGCGCA11950.020.2696349
GAGGTGT47750.019.6943596
TAAGGCT25950.019.4030234
ACTGTGT36850.019.3329188
TAATACT67600.018.3398044
CTGTCCG16250.018.120999
GGTCTAG16900.017.9911021
GTATAGG42350.017.948621
ACTGCGC13800.017.896498
AGAGGTG59750.017.8078295
AAGACTG29800.017.6932495
ATACTGT44150.017.6426226
TAGACTG23400.017.4575585
AGACTGT25400.017.3899866
Produced by FastQC (version 0.11.2)