FastQCFastQC Report
Sun 3 Sep 2017
CAWHCACXX_l03n01_osat_131.3510000009bb15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWHCACXX_l03n01_osat_131.3510000009bb15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13705220
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTG66000.018.7120255
ACTGTGC66650.017.1020668
TATACTG82750.016.9334475
TAGACTG44700.016.7896235
GTATAGG64650.016.2398381
ACAGTGC75650.015.56978
CCTATAC63450.015.2717893
GTGTAGG64100.015.0451311
TAGACAG46900.014.9892625
CTATACT88550.014.8587614
TACACAG60150.014.3723285
CTACACT67800.014.1518444
TACACCG50750.014.1329325
ACTGTCC43300.013.8203858
ATACTGT77000.013.8165476
ACAGTCC39500.013.7070898
GTCTAGG42550.013.509641
CCTAGAC41600.013.2451243
CTGTGCA91200.013.1754289
ACAGCGC48950.013.0984118
Produced by FastQC (version 0.11.2)