FastQCFastQC Report
Sun 3 Sep 2017
CAWHCACXX_l03n01_ogla_56.3510000009bceb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWHCACXX_l03n01_ogla_56.3510000009bceb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32727762
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG158150.017.2425821
TATACTG200400.016.2125855
CCTATAC150600.016.0225893
TACACTG144300.015.9979325
CTATACT217300.015.2577194
TAGACTG110650.015.1107215
CTACACT140000.014.7589394
GTATATA442100.014.4746051
ACTGTGC142350.013.9795358
TATACCG114600.013.9267115
TATACAG192850.013.8177435
GTATTGG214150.013.7985021
GTGTAGG128450.013.6844461
GTGTATG206400.013.5110161
TCTATAC170400.013.4360423
CCTACAC121650.013.4319873
GTATAAG158800.013.402541
ACAGTGC160450.013.1425448
TACACAG144600.013.1068565
TAGACAG118400.013.0384375
Produced by FastQC (version 0.11.2)