FastQCFastQC Report
Sun 3 Sep 2017
CAWHCACXX_l03n01_ogla_54.3510000009bd70.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWHCACXX_l03n01_ogla_54.3510000009bd70.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32790644
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG201800.017.1830085
GTATAGG139600.014.9737641
GTATTGG275250.014.6535911
TATACCG98000.014.6378875
GTATAAG157950.014.1064231
TAGACTG102900.014.0331695
CCTATAC138700.013.86998753
TTATACT252400.013.7005984
CTATACT209500.013.6946334
GTATATA443500.013.59351351
GTATAGA180150.013.3438141
TAGACAG113200.013.2598365
ACTGTGC135500.013.2492438
TCTATAC166850.013.1526493
TACACCG103250.013.0194865
GTGTAGG137050.012.7911931
TATACAG175550.012.7443485
TACACAG136000.012.6434965
GTATTAT254300.012.3859961
TGCGTTC190850.012.36811359
Produced by FastQC (version 0.11.2)