FastQCFastQC Report
Sun 3 Sep 2017
CAWHCACXX_l02n02_osat_152.3520000009ba57.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWHCACXX_l02n02_osat_152.3520000009ba57.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35802246
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG225450.024.3436091
CTATACT272050.020.3434624
CCTATAC239950.019.6405833
TATAGGG216400.018.7719732
TATACTG214450.017.6096675
CCCTATA223150.016.458252
TATACTA365300.015.7472415
GTGTAGG158700.015.0606691
TACACTG166300.014.9103645
TAGACTG125150.014.8787095
TATACCG117650.014.8178155
ACTGTGC169550.014.7343398
ATAGGGA225300.014.5917823
GTATATA480700.014.4420651
TAGGGAT236450.014.2447414
TACACAG156850.014.2035965
GTATTGG223450.014.0564071
ACAGTGC201300.013.5900618
GTATAAG156050.013.5503171
GTATAGA182550.013.5355131