FastQCFastQC Report
Sun 3 Sep 2017
CAWHCACXX_l02n02_osat_115.3520000009ba3d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWHCACXX_l02n02_osat_115.3520000009ba3d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35195592
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG216250.017.375025
GTATAGG169400.016.4375321
CTATACT228450.016.0119174
GTATATA467700.015.7781651
ACTGTGC168850.015.2172568
TATACAG222450.015.1611055
GTATTGG265000.014.9007321
CCTATAC166000.014.8531893
TAGACTG119450.014.83295255
TATACCG118850.014.7079965
TACACTG174400.014.5172925
TCTATAC175300.014.3362053
GTATAAG155650.014.3177941
TAATACT254100.014.1712554
TTATACT268300.013.9170024
ACAGTGC186300.013.84298
GTATAGA186350.013.8204541
TAGACAG125250.013.804755
GTATTAT243800.013.3508611
GTATTAG159200.013.2523331
Produced by FastQC (version 0.11.2)