FastQCFastQC Report
Sun 3 Sep 2017
CAWHCACXX_l02n02_ogla_61.3520000009bbb2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWHCACXX_l02n02_ogla_61.3520000009bbb2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57542350
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG282950.018.036341
TATACTG336800.016.9808985
TATACCG190150.016.3875395
CCTATAC284400.015.9860463
CTATACT369250.015.9659544
TAGACTG184600.015.0789865
GTATATA722650.014.7027191
TACACTG257200.014.5532995
GTATTGG395900.014.1628411
GTATAAG251200.013.9790811
GTGTAGG233250.013.8121671
GTATAGA295700.013.6430091
TACACCG200550.013.5955085
CCTAGAC182650.013.3951093
TAGACAG204850.013.3333155
ACTGTGC251000.013.2453378
TCTATAC276850.013.2131053
TATACAG319150.013.0679035
GTGTATG352500.012.9679031
TTATACT410400.012.8371254