FastQCFastQC Report
Sun 3 Sep 2017
CAWHCACXX_l02n02_ogla_60.3520000009bc3b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWHCACXX_l02n02_ogla_60.3520000009bc3b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55387657
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG360900.016.913225
TATACCG182550.016.1331795
GTATTGG449200.015.3699891
GTATAGG254950.015.0779121
TACACCG181550.014.5998355
CCTAGAC190200.014.5119673
GTATAAG250950.014.3336361
CTATACT339550.014.1726254
GTATATA736600.014.1531331
GTGTAGG233600.013.9336671
TAGACTG176950.013.9324295
ACTGTGC243850.013.8090858
TATACAG311050.013.7901055
TACACTG264950.013.6795145
GTCTAGG220700.013.5208811
TAGACAG202000.013.4510055
TACACAG239850.013.427655
GTATAGA297300.013.4095681
CGCCGTA260700.013.40951694-95
TGGTCGC586950.013.34025694-95