FastQCFastQC Report
Sun 3 Sep 2017
CAWHCACXX_l02n02_ogla_59.3520000009bcb1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWHCACXX_l02n02_ogla_59.3520000009bcb1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33670899
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG228550.016.4193025
GTATAGG158050.015.7236051
GTATTGG295800.015.662151
GTATAAG150350.015.2963161
CCTATAC158700.014.7580993
CTATACT226000.014.6720894
TACACTG167500.014.63334755
TATACCG110750.014.5829035
TACACCG118250.014.140035
GTGTAGG147400.014.1195741
TAGACTG114050.013.7861025
TATACAG199450.013.7420215
TAGACAG128150.013.6778115
GTATAGA183600.013.6390111
TCTATAC175700.013.6275943
CGCCGTA152750.013.43320894-95
GTATTAG170500.013.3492251
GTATATA490000.013.2755431
TATTGGG333300.013.2570072
CCTAGAC114000.013.2520393