FastQCFastQC Report
Sun 3 Sep 2017
CAWHCACXX_l02n02_ogla_26.3520000009baa7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWHCACXX_l02n02_ogla_26.3520000009baa7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23800645
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG148550.017.4275425
GTATAGG102650.015.9243751
TATACCG85950.015.8063815
GTATTGG258350.015.1007181
GTATATA322450.014.5451321
CTATACT150950.014.1622964
GTATAAG109950.013.9162911
TAGACTG74400.013.1524485
TTATACT183950.012.912924
CCTATAC103000.012.822613
TATTGGG290100.012.7749072
ACTGTGC105500.012.6502098
GTATAGA137200.012.6069541
TGCGTTC186550.012.5770099
TATACAG139050.012.5715585
GTATTAG124100.012.4827051
TACACTG104950.012.3564165
ATTATAC171100.012.3560283
TCTATAC119250.012.3103093
GGTGCGT208000.012.284657
Produced by FastQC (version 0.11.2)