FastQCFastQC Report
Sun 3 Sep 2017
CAWHCACXX_l02n02_ogla_25.3520000009bb3c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWHCACXX_l02n02_ogla_25.3520000009bb3c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18492819
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG119650.015.8005995
TATACCG66550.014.9176845
CCTATAC85100.014.9050763
TAGACTG62450.014.6040045
GTATAGG82800.014.40361
GTATATA258100.014.39615251
GTATTGG164500.014.00888251
CTATACT123950.013.9506114
GTATAAG88600.013.94335651
TATACAG107700.013.8048535
GTATTAG96100.013.2012651
CCTAGAC60650.013.15924553
ACTGTGC80450.013.1060728
GTATAAT132900.012.9422941
TACACTG86100.012.9096925
TCTATAC97000.012.78266053
GTATTAC82550.012.7204621
TAATACT147800.012.6315464
TACACCG62100.012.6210515
ATAATAC139300.012.379643
Produced by FastQC (version 0.11.2)