FastQCFastQC Report
Sun 3 Sep 2017
CAWHCACXX_l02n01_osat_152.3510000009ba5a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWHCACXX_l02n01_osat_152.3510000009ba5a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35802246
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG227200.022.8375971
CTATACT279400.019.4014994
CCTATAC237250.018.8634323
TATAGGG211900.017.7794482
TATACTG223650.017.4401535
ACTGTGC174800.015.813478
TATACTA370600.015.6654125
CCCTATA221450.015.6182282
TAGACTG122850.015.1595745
GTATTGG220650.014.9448531
TATACCG119700.014.6456375
GTGTAGG159100.014.51450351
TACACCG125250.014.4139135
TATACAG214100.014.2904285
TACACTG175150.013.9963775
ACTGTCC114450.013.8604178
TACACAG163550.013.8561915
GTATATA484900.013.7970611
CTACACT172400.013.7787174
GTATAAG161900.013.7058551
Produced by FastQC (version 0.11.2)