FastQCFastQC Report
Sun 3 Sep 2017
CAWHCACXX_l02n01_osat_115.3510000009ba30.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWHCACXX_l02n01_osat_115.3510000009ba30.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35195592
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG225050.018.2390865
CTATACT223950.016.3133684
GTATAGG167650.015.7297791
TATACAG221750.015.125495
TACACTG172150.015.0955415
GTATATA477350.014.950861
ACTGTGC167050.014.8978938
TAATACT253250.014.7824074
CCTATAC169400.014.6955633
TAGACTG121800.014.5098875
TTATACT273300.014.4975574
GTATTGG269600.014.4519731
TATACCG118500.014.2724985
TAGACAG128400.014.1340545
GTATTAT247350.013.8694251
TACACAG162850.013.857165
TCTATAC173750.013.8081323
GTATTAG162800.013.6008091
CTACACT172400.013.558024
GTATAAG153850.013.4037221
Produced by FastQC (version 0.11.2)