FastQCFastQC Report
Sun 3 Sep 2017
CAWHCACXX_l02n01_ogla_61.3510000009bbb5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWHCACXX_l02n01_ogla_61.3510000009bbb5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57542350
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG286550.017.262081
TATACTG330900.016.3101755
CTATACT372250.016.1448084
CCTATAC290800.016.0433183
TATACCG183200.015.7672225
TAGACTG183850.015.0396045
GTATAAG256800.014.7285781
TACACTG259950.014.3468625
TATACAG315750.014.1286075
ACTGTGC251250.013.8748958
GTATATA726050.013.8219771
GTATAGA290250.013.7842341
TACACCG196400.013.5222015
TACACAG239650.013.4607555
GTGTAGG237550.013.3617771
TAGACAG205700.013.1418065
GTATTGG389600.012.8059891
GTCTAGG191400.012.7107391
TATACTA516100.012.5930035
TCTATAC269600.012.5116783