FastQCFastQC Report
Sun 3 Sep 2017
CAWHCACXX_l02n01_ogla_60.3510000009bc3e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWHCACXX_l02n01_ogla_60.3510000009bc3e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55387657
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG360550.015.9044465
TATACCG183900.015.8105315
GTATAGG253050.015.2659461
GTATAAG246350.015.2375131
GTATTGG442350.014.8235631
CCTAGAC199350.014.2992023
TATACAG314750.014.188585
TAGACTG178550.014.1822185
ACTGTGC240350.013.9707068
TACACTG259750.013.9372215
GTGTAGG241100.013.7364691
TACACCG186750.013.635815
CTATACT340850.013.6317724
GTATATA729500.013.5285331
CCTATAC258300.013.1877753
GTATAGA298100.013.0067181
TAGACAG201850.012.9687955
TTATACT398800.012.889884
TACACAG233800.012.8424935
GTCTAGG219900.012.8135371