FastQCFastQC Report
Sun 3 Sep 2017
CAWHCACXX_l02n01_ogla_26.3510000009baaa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWHCACXX_l02n01_ogla_26.3510000009baaa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23800645
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG154750.016.3332045
GTATTGG263550.015.9384251
TATACCG89050.014.8854075
TATACAG135100.014.7701325
GTATATA329100.013.8178421
GTATAGG103550.013.8125091
GTATAAG113350.013.4567341
TATTGGG290950.013.4228232
TAGACTG77650.013.3384525
ACTGTGC100450.013.2863128
CTATACT152000.013.25295454
TTATACT187700.013.1369194
TGCGTTC192550.013.0241249
ATTATAC172350.012.9837473
TCTATAC125450.012.952273
GGTGCGT210550.012.9480617
TACACAG101050.012.6945675
GTGCGTT210550.012.6548128
GTATTAT182250.012.593191
TACACCG76600.012.5909275